Washington: In new research, scientists have found that there could be new treatments on the horizon for treating ‘diffuse intrinsic pontine glioma’, or DIPG, a devastating form of brain cancer that afflicts young children and is currently incurable.
The research, published in Proceedings of the National Academy of Sciences, shows that the drug acts on cellular cholesterol pathways, and suggests that these pathways may be fruitful targets for treating a variety of brain cancers.
Recent experiments in animal models of the disease have identified an experimental drug that effectively destroys DIPG cells and a team of scientists just figured out how this promising compound works.
DIPG tumours are located in the pons, a highly sensitive structure that connects the brain to the spinal cord. Surgical removal of tumours is effectively impossible since it poses the risk of fatal brain damage.
And although radiation can be used to temporarily reduce symptoms, cancer inevitably grows, with an average survival rate of less than one year. Which is to say: there is a pressing need for new ways to treat children with the disease.
A major development happened in 2014 in which a team of researchers showed that a compound known as MI-2 stops tumour growth in a mouse model of DIPG.
The drug was already on scientists’ radar for the treatment of leukemia, and was known to work on leukemia cells by interacting with menin, a protein that regulates gene expression.
So when Allis’ team began investigating the effect of MI-2 on DIPG cells, they initially suspected that it would work in a similar manner.”Our first hypothesis was that the drug switched off genes by interacting with menin,” says Richard Phillips, a researcher.
“But as we probed a little bit further, many of the things that we would expect to see didn’t pan out,” he added.
For example, when the researchers genetically removed menin from glioma cells, those cells remained sensitive to MI-2, indicating that the compound exerted its effects via a pathway distinct from that observed in leukemia.
The scientists then discovered that DIPG cells exposed to MI-2 failed to maintain healthy levels of cholesterol, and quickly died; but the cells could be rescued with a dose of supplemental cholesterol–suggesting that, in the case of glioma, MI-2 works by depleting the nutrient.
Eventually, the researchers discovered that MI-2 directly inhibits lanosterol synthase, an enzyme involved in cholesterol production.
The researchers also found that, while MI-2 destroys glioma cells, the drug doesn’t damage normal brain cells. This finding is consistent with other research showing that some cancer cells are particularly vulnerable to cholesterol disturbances.
This study contributes to a growing body of research pointing to cholesterol interference as a promising new way to treat cancer.
“Some existing drugs, initially made for people with high cholesterol, were designed to target lanosterol synthase–but they were never really thought of as cancer drugs.One of them is even more potent than MI-2, so we’re now working with a team of chemical biologists to see if we can modify the drug so that it reaches the brain,” he said.
More broadly, this research highlights the importance of knowing not just that a drug works, but how it works. In this case, the discovery that MI-2 acts on lanosterol synthase revealed that DIPG tumours are sensitive to cholesterol interference- a finding that opens avenues for the production of even more effective compounds.
“You can’t assume that what it says on the label is actually how a drug works,” added Phillips.